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human antibody against ago2  (Proteintech)


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    Proteintech human antibody against ago2
    Human Antibody Against Ago2, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human antibody against ago2/product/Proteintech
    Average 96 stars, based on 184 article reviews
    human antibody against ago2 - by Bioz Stars, 2026-03
    96/100 stars

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    miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between <t>anti-Ago2-mediated</t> complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001
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    miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between <t>anti-Ago2-mediated</t> complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001
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    miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001

    Journal: Bioengineered

    Article Title: Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)

    doi: 10.1080/21655979.2021.1984002

    Figure Lengend Snippet: miR-640 was downregulated in RCC and correlated with circ_0054537 via targeting. (a, b) GSE61741 compared miRNAs levels including miR-640 in peripheral bloods between renal cancer patients and normal controls. (c, e) RT-qPCR detected miR-640 level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (d) Pearson’s correlation analysis analyzed the correlation between circ_0054537 and miR-640 levels in RCC tissues. (f) The predicted miR-640-binding sites in circ_0054537-WT were mutated. (g, h) Dual-luciferase reporter assay system compared luciferase activity of circ_0054537-WT/MUT vectors between miR-con mimic (miR-con) transfection and miR-640 mimic (miR-640) transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of circ_0054537 and miR-640 between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) RT-qPCR compared miR-640 level between si-con-transfected 786-O and A498 cells and si-circ_0054537 #1 -transfected above cells. *** P < 0.001

    Article Snippet: Protein G Sepharose 4 Fast Flow bead slurry (GE Healthcare, Beijing, China) was pre-coated with antibody against human Ago2 (anti-Ago2; ab32381, 1:50; Abcam) or the negative control anti-IgG (ab109489, 1:100; Abcam).

    Techniques: Quantitative RT-PCR, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection

    NPTX2 was upregulated in RCC and targeted by miR-640. (a) GEPIA showed NPTX2 expression on box plots in kidney renal clear cell carcinoma (KIRC, also named as ccRCC) tumor tissues and normal tissues. (b) RT-qPCR detected NPTX2 mRNA level in tissues from 39 RCC patients. (c) Pearson’s correlation analysis analyzed the correlation between miR-640 and NPTX2 mRNA levels in RCC tissues. (d, e) Western blotting detected PTX2 protein level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (f) The predicted miR-640-binding sites in NPTX2-3ʹUTR-WT were mutated. (g, h) Dual-luciferase reporter assay compared luciferase activity of NPTX2-3ʹUTR-WT/MUT vectors between miR-con transfection and miR-640 transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of miR-640 and NPTX2 mRNA between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) Western blotting compared NPTX2 protein level between in-miR-con transfection and in-miR-640 transfection in 786-O and A498 cells. ** P < 0.01 and *** P < 0.001

    Journal: Bioengineered

    Article Title: Silencing circular RNA circ_0054537 and upregulating microRNA-640 suppress malignant progression of renal cell carcinoma via regulating neuronal pentraxin-2 (NPTX2)

    doi: 10.1080/21655979.2021.1984002

    Figure Lengend Snippet: NPTX2 was upregulated in RCC and targeted by miR-640. (a) GEPIA showed NPTX2 expression on box plots in kidney renal clear cell carcinoma (KIRC, also named as ccRCC) tumor tissues and normal tissues. (b) RT-qPCR detected NPTX2 mRNA level in tissues from 39 RCC patients. (c) Pearson’s correlation analysis analyzed the correlation between miR-640 and NPTX2 mRNA levels in RCC tissues. (d, e) Western blotting detected PTX2 protein level in 39 RCC patients’ tissues and cells (HK-2, 786-O and A498). (f) The predicted miR-640-binding sites in NPTX2-3ʹUTR-WT were mutated. (g, h) Dual-luciferase reporter assay compared luciferase activity of NPTX2-3ʹUTR-WT/MUT vectors between miR-con transfection and miR-640 transfection in 786-O and A498 cells. (i, j) RIP assay detected RNA enrichment of miR-640 and NPTX2 mRNA between anti-Ago2-mediated complex and anti-IgG-mediated complex. (k) Western blotting compared NPTX2 protein level between in-miR-con transfection and in-miR-640 transfection in 786-O and A498 cells. ** P < 0.01 and *** P < 0.001

    Article Snippet: Protein G Sepharose 4 Fast Flow bead slurry (GE Healthcare, Beijing, China) was pre-coated with antibody against human Ago2 (anti-Ago2; ab32381, 1:50; Abcam) or the negative control anti-IgG (ab109489, 1:100; Abcam).

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Binding Assay, Luciferase, Reporter Assay, Activity Assay, Transfection